southern blotting vs northern blotting vs western blotting

In this method the RNA is extracted right from the presented sample. On the next we will be discussing about the northern blotting! This method was developed by the Alwine and his colleagues in 70’s. This method in short gives out the complete information on the concept of DNA size and identification. This whole method will be coming up with the production of the nitrocellulose filter with the means of DNA fragments that is all transferred to the filter. At that point solution is made that is used at the stage of passing through the gel and even filtering the paper towel.

After sometime these bands will be transferred to nitrocellulose filter. You can even make these bands visible by the method of some specific staining. Then they will be transformed into bands that are included with so many copies of that DNA. As soon as these all fragments are separated by the way of using the process of electrophoresis. Afterwards it is all cut into fragments all the way by using the assistance of the restriction enzyme. In southern blotting is all about the DNA that is hence all extracted from the human cell. Southern blotting was developed for the very first time by the E.M. It is all extracted from a tissue and radioactively labeled. In the process of the Northern blotting the substrate nucleic acid is considered to be the gathering of the isolated DNA fragments. Well the main difference that is witnessed in this method is related to the addition of the addition of formaldehyde in the agarose gel that performs the role of denaturant. On the contrary side Northern blot is defined to the method that is much used in the molecular biology research just for the sake to carry out the study work of the gene expression. They use these methods just to transfer the size-separated DNA right into the filter membrane for probe hybridization. Southern blotting is the combination of the agarose gel electrophoresis in support of the size separation of DNA in the company of some methods. Its main purpose is to check in support of the presence of a DNA sequence in a DNA sample. Southern Blotting is a process that is on the normal basis carried away by the use of the molecular biology. But on other side the northern blotting is a process that is used for the purpose of the detecting RNAs that is all found to be separated by the process of electrophoresis. Do you know what is the main difference between the Southern and Northern Blotting? Southern blotting is defined to be a method that is mainly used at the time when we want to search out for the appearance of any sort of particular DNA fragment in a given sample. Therefore right in this article we will be explaining the complete concept about the comparison of and Northern Blotting, their working concepts and their main differences. Some of the people are not able to figure out the actual difference between the Southern and Northern Blotting easily.

Southern blotting vs northern blotting vs western blotting